A real-world study on Clofarabine and Cytarabine combination in patients with relapsed/refractory acute myeloid leukemia

A Real-World Study on Clofarabine and Cytarabine Combination in Patients with Relapsed/Refractory Acute Myeloid Leukemia

Valeria Crobu1, Giovanni Caocci2, Giorgio La Nasa2, Laura Saderi1, Giovanni Sotgiu1 and Claudio Fozza1.

1 Department of Medical, Surgical and Experimental Sciences, University of Sassari, Sassari, Italy.
2 Hematology Unit, Department of Medical Sciences, University of Cagliari, Cagliari, Italy.

Correspondence to: Professor Claudio Fozza, Department of Medical, Surgical and Experimental Sciences, University of Sassari, Viale San Pietro 12, 07100 Sassari, Italy. Telephone and fax number + 39 079 228282. Email address: cfozza@uniss.it.   

Published: May 1, 2019   
Received: January 8, 2019                     
Accepted: March 8, 2019
Mediterr J Hematol Infect Dis 2019, 11(1): e2019032 DOI 10.4084/MJHID.2019.032

This is an Open Access article distributed under the terms of the Creative Commons Attribution License
https://creativecommons.org/licenses/by-nc/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background and Objective: While tyrosine kinase inhibitors (TKIs) have transformed CP-CML management, limited data exist on their use in clinical practice.
Methods: SIMPLICITY (NCT01244750) is an observational study in CP-CML patients, exploring first line (1L) TKI use and management patterns in the US and Europe. Over half of the patients recruited in Europe are from Italy (n=266). This is an analysis of the Italian cohort and a comparison with the rest of the European SIMPLICITY population. Baseline demographic, factors influencing the choice of first-line TKI, response monitoring patterns and predictors of monitoring, and treatment interruptions, discontinuations and switching by index TKIs are presented for the Italian cohort in the first year of treatment and compared with that for the overall European SIMPLICITY cohort.
Results: Italian patients received 1L imatinib (IM; retrospective [(n=31]; prospective [n=106]), dasatinib (DAS; n=56) or nilotinib (NIL; n=73). Documented cytogenetic response monitoring by 12 months was lower than expected, but almost all patients had documented molecular response monitoring. Fewer patients discontinued first-line TKI by 12 months in Italy compared with the rest of the European SIMPLICITY population (p=0.003). Of those with ≥12 months follow-up since the start of 1L TKI, only 7.1% (n=19) of Italian patients switched to a second-line TKI, a third less than in the rest of the European SIMPLICITY population. Of interest, intolerance as opposed to resistance, was the main reason for switching.
Conclusions: This analysis provides valuable insights into management and treatment patterns in Italian patients with CML within routine clinical practice.


Long-term survival has increased in patients with acute myeloid leukemia (AML) over the last two decades due to improved supportive care and improved access to allogeneic transplantation than to substantial improvement of chemotherapy effectiveness. In particular, patients with relapsed/refractory (R/R) AML, who usually have an unfavorable prognosis, should undergo allogeneic transplantation after a second complete remission (CR2) to potentially achieve a treatment success.[1] Unfortunately, only a few R/R AML patients can be treated with intensive therapy and the probability of remission after salvage is significantly lower than after induction therapy. However, the CR rate is 55% in patients aged between 16 and 49 years and even lower (20-30%) in older cohorts. Additionally, only 66% of patients aged 16-49 years who achieve CR are eligible to allogeneic transplantation with a 5-year overall survival (OS) of 40%. The transplantation rate is even lower in older patients, and worst outcomes (3-year OS: ~10%) can be associated not only with increasing age and comorbidities but also with unfavorable cytogenetic and molecular profiles. Factors associated with poor prognosis are short CR1 duration, older age at the time of relapse, unfavorable cytogenetics at diagnosis and relapse after allogeneic transplantation. It is worth noting that mortality rate remains high among patients who undergo allogeneic transplantation in CR2, due to high transplant-related mortality (TRM) and relapse rate. The outcome of patients who relapse after allogeneic transplantation is particularly poor and the earlier is the relapse, the worse is the outcome. In this context, other factors associated with poor outcome are age >40 years, active graft versus host disease (GvHD), unfavorable cytogenetics at diagnosis, matched unrelated donor (MUD) or cord blood donor.[2-4]
Given the lack of a standardized strategy for R/R AML and its disappointing prognosis, the enrollment in clinical trials is still considered the best option. Nonetheless, only a minority of patients can be recruited based on restrictive selection criteria and the enrollment of specialized centers. Chemotherapy-based salvage regimens are usually prescribed to patients with R/R AML. Most of the protocols include the combination of high doses of Cytarabine with other agents, such as anthracyclines, Etoposide, and nucleoside analogs. The most frequently administered combinations are Fludarabine, Cytarabine and Idarubicine (FLAI) or Mitoxantrone, Etoposide and Cytarabine (MEC). Recently, a systematic literature review showed a relatively high CR rate (44-60%), a short CR duration (5-10 months) and OS (6-9 months) in individuals exposed to standard chemotherapy protocols.[1]
The efficacy of the nucleoside analog Clofarabine has been assessed in patients with AML both in monotherapy and in combination, specifically with Cytarabine based on their synergistic action.[5] In particular, administration of Clofarabine with high doses of Cytarabine (CLARA) showed a CR rate of 44% and a mean OS of 6 months in R/R AML patients, together with a favorable safety profile.[6] In case of prescription of CLARA as a bridge to allogeneic transplantation, a median OS of 24 months and a 3-year OS of 55% can be achieved. Furthermore, CR can also be obtained in patients who were non-responders to Fludarabine-containing regimens.[7-8]
We retrospectively evaluated 47 and 22 patients with R/R AML consecutively exposed between 2001 and 2017 to second- and third-line therapy, respectively, in the Hematology units of the University Hospitals of Sassari and Cagliari. The aim of this observational study was to compare effectiveness, i.e., overall response rate (ORR), OS and relapse-free survival (RFS), and safety profile of CLARA and other standard chemotherapy protocols. In the cohort exposed to second-line therapy, 15 received CLARA and 32 different chemotherapy regimens (control group), whereas 7/22 and 15/22 were treated with CLARA or with other regimens in the cohort of the third-line therapy, respectively. No specific criteria were applied to select either CLARA or different therapeutic regimens, but all cases were discussed collegially taking into consideration previous lines of therapy and expected toxicities. All therapeutic approaches were allied with a primary intention to carry on with transplant after reaching CR. Dosages of Clofarabine in the CLARA regimen ranged from 22.5 to 40 mg/m2, followed 4 hours later by a dose of Cytarabine from 1 to 2 g/m2 for 5 days. The most frequently prescribed chemotherapy schemes in the control group were: Fludarabine-Cytarabine-based regimens with or without anthracycline (21 and 1 patients on second- and third-line therapy, respectively) and Cytarabine-anthracycline-Etoposide-based combinations (10 and 6 patients on second- and third-line therapy, respectively). Other less frequently administered regimens were based on Gemtuzumab ozogamicin, with or without chemotherapy (5 cases on third-line treatment). Comparisons between the groups mentioned above were carried out with chi-squared or Fisher's exact tests for qualitative variables and Student's t-test distribution or Mann-Whitney's tests for parametric and nonparametric variables. All analyses were performed with the statistical software Stata version 15 (StatsCorp, Texas).
As shown in tables 1 and 2, the median (IQR) age of the entire cohort was 53 (33-64) years. According to the 2016 World Health Organization (WHO) classification, among patients treated in second line 25 patients (53%) had not otherwise specified AML (AML-NOS), 15 (32%) AML with myelodysplasia-related changes, 3 (6%) therapy-related AML, 2 (4%) AML with t(8;21) and 2 (4%) AML with NPM1 mutation. Among patients treated in third line 10 patients (45%) had AML-NOS, 8 (36%) AML with myelodysplasia-related changes, 1 (5%) therapy-related AML, 1 (5%) AML with t(8;21) and 2 (9%) AML with NPM1 mutation. According to the 2017 prognostic stratification by European Leukemia Net (ELN) 8 (12%), 38 (55%), and 23 (33%) patients were classified as at low, intermediate, and high-risk, respectively. Thirty-two (46%) had relapsed AML, whereas 37 (54%) had a primary refractory disease. No statistically significant differences were found between CLARA and control group according to age, WHO subtypes, ELN classification, and disease status in patients treated in both second and third line. We specifically evaluated the potential impact of latency between remission and relapse in each group, but no significant differences could be detected. In fact, in patients treated in second line median (IQR) lag between remission and relapse was 2 and 0 months in CLARA and control group (p-value: 0.60), respectively while in patients treated in the third line it was equal to 0 months in both groups ((p-value: 0.10).

Table 1 Table 1. Characteristics and outcomes of patients treated in second line

Table 2 Table 2. Characteristics and outcomes of patients treated in third line

As shown in table 1, CR rate in the second-line therapy cohort was 53% and 28% in the CLARA and control (p-value: 0.09), respectively, while ORR was 67% and 44% (p-value: 0.14). Median OS was 8 and 7 months (p-value: 0.98) in the CLARA and the control group, respectively (figure 1); on the other side, median RFS was 3 and 0 months (p-value: 0.02), respectively. As shown in table 2, CR rate in the third-line therapy cohort was 29% and 13% in the CLARA and control (p-value: 0.57), respectively, while ORR was 29% and 20% (p-value: 1.0). Median OS was 2 and 3 months (p-value: 0.52), respectively. A median RFS of 0 was recorded in both groups. Patients who received allogeneic transplantation after second-line therapy were 20% and 6% in the CLARA and control group (p-value: 0.31), respectively while patients who received allogeneic transplantation after third-line therapy were 29% and 20% in the CLARA and control group (p-value: 1.00), respectively. Among the patients who underwent allogeneic SCT after second-line treatment, out the 2 treated within the control group one was in CR, and one in CR with incomplete recovery (CRi) whereas out the 3 treated with CLARA 2 were in CR and one in partial remission (PR). All of them achieved CR after transplant, and transplant-related mortality (TRM) was 0%. Among the patients who underwent allogeneic SCT after third-line treatment, out the 3 treated within the control group one was in CR, and two had a refractory disease (RD) whereas the 2 patients treated with CLARA were both in CR. Among the 3 patients treated within the control group, 2 achieved CR after transplant, and one still showed RD whereas among the 2 patients treated with CLARA one confirmed his CR while one died soon after transplant for TRM. Safety profile was good, with a tendentially higher rate of reversible (i.e., <2 weeks) hepatotoxicity cases after second-line therapy in the CLARA group (33% VS. 9%; p-value: 0.09) and a higher rate of pneumonia after third-line therapy in the CLARA group (57% VS. 13%; p-value: 0.05).

Figure 1 Figure 1. a) Kaplan Mayer survival curves for patients treated in second line in the overall population b) Kaplan Mayer survival curves for patients treated in second line in the population stratified according to their treatment with CLARA or with the control group 

Comparison of our findings with those described in the literature showed that CR and OS rates were similar, whereas RFS was slightly lower in our cohort. Potential explanations could be the low percentage of patients exposed to chemotherapy as a bridge to allogeneic transplantation, as well as the retrospective design of our study which selected patients tendentially less fit than those described in prospective studies. Our data substantially confirm the potential effectiveness of CLARA in patients with R/R AML. In fact, even though a statistically significant improvement over other frequently prescribed salvage regimens was not demonstrated, our findings suggest a potential positive trend in terms of improvement in both CR, ORR, and RFS within a real-life setting. When considering that the main goal in R/R AML, regardless of salvage regimens, is to perform allogeneic transplantation as earliest as possible, CLARA may represent a valid option in this setting especially in patients with a contraindication for or resistant to anthracycline-containing regimens.


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