Original Articles
Vol. 8 (2016): Review, Original Articles, Case Reports
MOLECULAR HETEROGENEITY OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE DEFICIENCY IN BURKINA FASO: G-6-PD BETICA SELMA AND SANTAMARIA IN PEOPLE WITH SYMPTOMATIC MALARIA IN OUAGADOUGOU
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Received: April 11, 2016
Accepted: May 25, 2016
Accepted: May 25, 2016
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Infectious Diseases,
Hematology,
Molecular Biology
Authors
The G-6-PD deficiency has an important polymorphism with genotypic variants such as 202A/376G, 376G/542T and 376G/968T known in West African populations. It would confer protection against severe forms of malaria although there are differences between the various associations in different studies. In this study we genotyped six (06) variants of the G-6-PD gene in people with symptomatic malaria in urban areas in Burkina Faso.
One hundred and eighty-two (182) patients who tested positive using rapid detection test and microscopy were included in this study. A regular PCR with the GENESPARK G6PD African kit was run followed by electrophoresis, allowing initially to genotype six SNPs (G202A, A376G, A542T, G680T, C563T and T968C). Women carrying the mutations 202A and/or 376G were further typed by real-time PCR using TaqMan probes rs1050828 and rs1050829.
In the study population the G-6-PD deficiency prevalence was 9.9%. In addition of G-6-PD A- (202A/376G) variants, 376G/542Tand 376G/968T were detected. Hemoglobin electrophoresis revealed that 22.5% (41/182) of the individuals had HbAC compared with 2.2% with HbAS and one individual had double heterozygous HbSC. There was no correlation between the G-6-PD deficiency or haemoglobinopathies and symptomatic malaria in this study.
As opposed to previous genotyping studies carried out in Burkina Faso, this study shows for the first time the presence of the variant A- (376G/968C) and warrants further investigation at the national level and in specific ethnic groups.
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Citations
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Original ArticleSupporting Agencies
African Economic and Monetary Union (WAEMU) (through the PACER2 program)How to Cite
“MOLECULAR HETEROGENEITY OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE DEFICIENCY IN BURKINA FASO: G-6-PD BETICA SELMA AND SANTAMARIA IN PEOPLE WITH SYMPTOMATIC MALARIA IN OUAGADOUGOU” (2016) Mediterranean Journal of Hematology and Infectious Diseases, 8, p. e2016029. doi:10.4084/mjhid.2016.029.
