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Platelet-rich plasma (PRP) is an inexpensive and safe substitute of recombinant growth factors in vitro and in vivo.
Due to its putative effect on tissue repair, the use of autologous PRP
has become largely popular. Recently, a jellified PRP derivative
obtained from umbilical cord blood (CB) has been utilized in vivo
to treat mucosal and cutaneous lesions. Nevertheless, whether PRP
derived from CB and adult blood display different potency in promoting
cell growth in vitro has been
rarely investigated. In this study, we compared cytokine profile and
mesenchymal cell growth supporting the ability of platelet lysate
obtained from adult and cord blood. Our in vitro results strongly back the utilization of CB platelet lysate in vivo,
as an efficacious, safe and inexpensive alternative to promote damaged
tissue healing when the autologous PRP is contraindicated. Moreover,
the policy of manufacturing CB platelet lysate can limit the current
disposal of many collected CB units not suitable for transplant due to
their low nucleated cell count.
Materials and Methods
Results and Discussion
|Figure 1. Mean values + SEM of cytokine levels in plasma and platelet lysates obtained from cord blood (yellow columns) and adult blood (red columns). (A) Inflammatory cytokines (B) Anti-inflammatory cytokines. * = p < 0.05; ** = p <0.01.|
|Figure 2. Mean values + SEM of cytokine levels in plasma and platelet lysates obtained from cord blood (yellow columns) and adult blood (red columns). (A) Chemokines (B) Growth factors * = p < 0.05; ** = p <0.01.|
|Figure 3. Cord blood and adult blood platelet lysates activity in MSCs cultures (mean values of three different experiments). A) Cumulative population doubling (PD) index in BM derived MSCS supplemented with 10% FBS (grey columns), or platelet lysates form adult (yellow columns) or cord (orange columns) blood. B) Number of CFU-F obtained in the same culture conditions; the representative image of the CFU-F culture after staining is shown under each corresponding culture condition. ** = p < 0.01.|