Peripheral Blood Cell Mitochondrial Dysfunction in Myelodysplastic Syndrome Can Be Improved by a Combination of Coenzyme Q10 and Carnitine
Kalman Filanovsky1*, Michal Haran1*, Vita Mirkin1, Andrei Braester2, Olga Shevetz1, Anfisa Stanevsky1, Erica Sigler1, Ekaterina Votinov1, Yehudit Zaltsman-Amir3, Alain Berrebi1, Atan Gross3 and Lev Shvidel1
1 Hematology Institute, Kaplan medical center, Rehovot, Israel, affiliated with the Hebrew University, Jerusalem, Israel.
2 Hematology institute, Galilee medical center, Nahariya, Israel.
3 Dept. of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel.
* Both authors equally contributed to this manuscript.
Received: June 20, 2020
Accepted: October 3, 2020
Mediterr J Hematol Infect Dis 2020, 12(1): e2020069 DOI 10.4084/MJHID.2020.072
| This is an Open Access article distributed
under the terms of the Creative Commons Attribution License
(https://creativecommons.org/licenses/by-nc/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
mitochondrial abnormalities and genetic aberrations in mitochondrial
proteins have been known in Myelodysplastic syndrome (MDS), yet there
is currently little data regarding MDS's metabolic properties and
energy production cells. In the current study, we used state-of-the-art
methods to assess OXPHOS in peripheral blood cells obtained from MDS
patients and healthy controls. We then assessed the effect of food
supplements- Coenzyme Q10 and carnitine on mitochondrial function and
hematological response. We show here for the first time that there is a
significant impairment of mitochondrial respiration in peripheral blood
cells in low-risk MDS, which can be improved with food supplements. We
also show that these supplements may improve the cytopenia and quality
Myelodysplastic syndrome (MDS) is a heterogeneous clonal disease of the bone marrow which is manifested by ineffective hematopoiesis and genomic instability leading to worsening cytopenias and a high percentage of transformation to acute myeloid leukemia (AML). The treatment options in MDS are quite limited.[4,5]
There are several lines of evidence suggesting the importance of mitochondrial dysfunction in the pathogenesis of MDS. First, structural mitochondrial abnormalities have been known for many years; Second, the importance of genetic aberrations in mitochondrial proteins in the prognosis of MDS and AML has been demonstrated by numerous studies looking at the gene and protein expression.[7-8] A recent study demonstrated early shedding of mitochondria from reticulocytes in a sub-group of MDS patients, showing impaired mitochondrial dynamics. However, there is currently little data regarding the metabolic properties and energy production of peripheral blood MDS cells. We thought that in view of treatment options that can improve mitochondrial energy production, it is crucial to develop such an assay.
Such a readily available and inexpensive option is Coenzyme Q10 (CoQ10). This vitamin-like compound is an essential component of the mitochondrial electron transport chain and plays a central role in mitochondrial oxidative phosphorylation and adenosine triphosphate (ATP) production. Significant improvement in mitochondrial respiration with a clinical improvement has been achieved by oral administration of therapeutic doses of CoQ10 in various diseases.[10,11] Further support for the role of impairment of mitochondrial function in the pathogenesis of the disease as well as a possible novel therapeutic approach was seen in a pilot study on low-risk MDS patients in which treatment with CoQ10 resulted in about 25% hematologic response and even cytogenetic response in some of the patients.
L-carnitine is required to transport fatty acids from the cytosol to the mitochondrial during the breakdown of lipids. It has also been used to improve mitochondrial function and has been shown to have a synergistic effect when combined with coenzyme Q10 in various medical conditions.[13–16]
Patients and methods
For optimal enhancement of mitochondrial function, patients were given a combination of Ultrasome™ (a better-absorbed formulation of CoQ10)-180 mg/day, L-carnitine-2000 mg/day, and a standard vitamin-mineral complex to ensure that there will be no lack of essential vitamins or minerals (a kind donation of Herbamed LTD, Israel), to be taken daily for six months. Patients were allowed to continue treatment for an additional six months if they desired to do so, and it was deemed beneficial by the protocol committee. Quality of life (QOL) was assessed using the FACT questionnaire at the beginning and third-month endpoint. We thought it was important to assess patient-reported outcomes alongside the objective parameters. Samples for assessing in vitro mitochondrial energy production in peripheral blood cells were taken in some of the patients before and after six months of treatment.
Endpoints were: hematological response according to IWG criteria and meaningful improvement in the quality of life and functional ability after three months of treatment with food supplements.
The study was approved by local IRB and registered at the National Institute of Health (NIH), registry no. NCT02042482.
Assessment of mitochondrial function. Mitochondrial function was assessed in peripheral blood cells using the Seahorse XF Analyzer, as previously described. The XF Analyzer creates micro-chambers of only a few microliters in specialized cell culture micro¬plates. This enables OCR (oxygen consumption rate) to be monitored in real-time. Briefly, cells were plated on a Cell-Tak™ coated 24 well XF V7 cell culture microplate at 0.5×106 cells per well in 50 μL of XF assay medium. Plates were centrifuged at 1200 rpm for 5 minutes to allow the cells to adhere. 450 ul XF assay medium containing 5mM glucose, 2mM pyruvate, and 2 mM Glutamax (Invitrogen) at pH 7.4 were added to each well. Cells were incubated for one hour without CO2 for equilibration before XF bioenergetic assay. Basal Oxygen Consumption Rate (OCR: picomoles of O2 per minute) was measured, followed by consecutive injections of the following: 1. Oligomycin (ATP synthase inhibitor); 2. the electron transport chain accelerator FCCP (5 µM gives the maximal OCR capacity of the cells); 3. The electron transport-chain-inhibitor rotenone and Antimycin A, which inhibit mitochondrial respiration.
We decided to perform all experiments on fresh peripheral blood cells, which were used to avoid the need for repeat bone marrow aspirations. This was based on the fact that since previous studies have shown that peripheral blood cells harbor the same mutations as less mature cells obtained from the bone marrow, thus making it likely that peripheral blood cells will harbor the same genetic aberrations which affect mitochondrial respiration. Blood cells were not separated, and we know that mostly neutrophils and platelets could contribute to the results.
In each experiment, samples from MDS patients were compared to samples obtained from matched volunteers without hematological disorders to control for inter-assay variability. The same controls were used for all samples obtained from the same patient.
Statistical analysis. Statistical analysis was performed using the paired student t-test. The significance level was set at p<0.05.
|Table 1. Clinical characteristics of the patients.|
|Figure 1 Significant improvement in Hemoglobin (Hb) level (red) and decreased need for Packed cell transfusion (blue) in a 56 year old man with MDS with ring sideroblasts following treatment with food supplements.|
The FACT questionnaire was administered to 18 patients before and three months endpoint. There was a significant improvement in the mean FACT score (126.3±26.9 points before treatment vs. 117.3±26.3 after treatment; p=0.011). Notably, the scores' improvement was mostly in the physical well-being domains (mean, 22.2 points vs. 18.5; p=0.03) and not in the social and emotional domains. Interestingly These results demonstrated a moderate, though significant improvement in overall physical ability and quality of life, even in those patients that did not have a discernible hematological response.
Peripheral blood cells obtained from MDS patients have significant impairment of mitochondrial respiration, which can be reversed with food supplements. Mitochondrial oxidative phosphorylation was assessed in peripheral blood cells obtained from 20 MDS patients. Each experiment was done on a patient matched to a healthy control and repeated three times. In most patients (18/20), there was a significant decrease in basal oxygen consumption rate and the spare respiratory capacity (see Figure 2a for a representative patient) compared to healthy controls. This result was statistically significant (see Figure 2b). Interestingly, there was an increased oxygen consumption rate in two patients with excess of blasts in bone marrow (see Figure 3 for a representative patient).
After six months of treatment, OCR assessment was done in five patients matched to their previous controls. The value pre-treatment was compared to the value post-treatment. A discernible improvement was seen in all the patients tested. The difference in OCR pre and post-treatment was statistically significant (p<0.05). (Figure 4).
Safety and tolerability of combination supplement treatment. The treatment with food supplements was well tolerated; most adverse events were transient gastrointestinal symptoms of grade 2 or less. Surprisingly, two patients who had more advanced disease with an excess of blasts in the bone marrow developed disease progression within the first few weeks of treatment. Those patients also had increased OCR before treatment. Interestingly, it was shown that AML cells had increased OCR, suggesting that increased OXPHOS may be the first step in developing AML. As we could not rule out the possibility that the food supplements augmented progression, we amended the protocol to exclude patients with excess (over 5%) of blasts. Another unexpected observation involved two patients who developed gastric diffuse large cell lymphoma one month and 30 months after cessation of treatment.
In the current study, utilizing novel tools, we directly show for the first time that mitochondrial energy production is indeed impaired in peripheral blood cells obtained from patients with low-risk MDS. We showed that both the basal OCR and the spare respiratory capacity were significantly decreased compared to age-matched healthy controls. Those finding could have both diagnostic and therapeutic implications. It may also provide a non-invasive tool for monitoring response to treatment. Furthermore, we showed that this impairment in mitochondrial respiration can be partially reversed with food supplementations known to augment mitochondrial function. In some of the patients, this was also accompanied by a hematological response.
Galili et al. demonstrated a clinical benefit of high dose CoQ10 in some MDS patients. Likewise, combined treatment with CoQ10 and carnitine ameliorated anemia and RBC-transfusion-dependence in 6 out of 28 (21.4%) in our patient group. This improvement is noteworthy, as generally, low-risk MDS patients have minimal treatment options once they do not respond to ESAs. Interestingly, although the hematological response was achieved only in some of the patients treated with supplements, patient-reported outcomes of overall strength and physical ability were increased in most of them, along with improvement in mitochondrial respiration of peripheral blood cells.
The treatment was generally well tolerated. An unexpected observation, which is not in line with the reported side effects of the used food supplements, was a relatively high prevalence of second hematological malignancies in our cohort. It is hard to discern in such a small series of patients whether this is related to the treatment or coincidental with it. Progression to AML in MDS patients is frequent; also, lymphoid malignancies may be more prevalent than in the general population, and gastric lymphoma cases in patients with MDS have been described. Moreover, it has been shown that peripheral blood lymphocytes obtained from MDS patients harbor pre-malignant mutations. Overall, this treatment seems to be safe and effective in most low-risk MDS patients. Caution should be taken in patients with excess of blasts. This finding is important as many times patients take food supplements without the awareness of their clinicians.
Our study's limitation was that it was not randomized and that food supplements were given for a relatively short period. Further studies with a much longer follow-up are required to assess the safety and efficacy, as well as timing of treatment with the food supplements in different sub-groups of MDS patients.
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