USP9X INCREASED TUMOR ANGIOGENESIS IN MANTLE CELL LYMPHOMA BY UPREGULATION OF CCND1- MEDIATED SOX11

Abstract

Mantle cell lymphoma (MCL) is an aggressive lymphoid malignancy with a poor prognosis. Ubiquitin-specific peptidase 9, X-linked (USP9X), has been found to be associated with multiple physiological pathways and regulate a variety of cellular activities. In this study, we explored the role of USP9X in MCL in vitro and in vivo. USP9X was verified to be increased in peripheral blood mononuclear cells (PBMCs) of MCL patients and MCL cells. Moreover, USP9X overexpression and knockdown were performed in MCL cells. We proved that USP9X overexpression promoted proliferation and cell cycle, and suppressed cell apoptosis in MCL cells. Unregulation of angiogenesis and cell migrate were induced by USP9X overexpression in MCL cells. However, USP9X knockdown showed opposite effects. In addition, USP9X was discovered to decrease Cyclin D1 (CCND1)-mediated SOX11 expression in MCL cells. We demonstrated that SOX11 overexpression reversed USP9X knockdown-mediated angiogenesis in MCL cells. Besides, tumor formation was inhibited by USP9X knockdown in mice in vivo. In conclusion, these results revealed that USP9X promoted tumor angiogenesis in MCL via increasing CCND1- mediated SOX11.